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ASCP MEDICAL LABORATORY TECHNICIAN - MLT(ASCP) : ASCP-MLT Exam

ASCP-MLT actual test
  • Exam Code: ASCP-MLT
  • Exam Name: MEDICAL LABORATORY TECHNICIAN - MLT(ASCP)
  • Updated: Aug 26, 2026
  • Q & A: 562 Questions and Answers
  • PDF Demo
  • PC Test Engine
  • Online Test Engine
  • Total Price: $59.99  

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ASCP ASCP-MLT Exam Syllabus Topics:

SectionWeightObjectives
Microbiology15-20%- Identification of pathogenic microorganisms
- Specimen collection and culture techniques
- Mycology, parasitology, and virology
- Bacteriology and antimicrobial susceptibility
Immunology and Serology5-10%- Infectious disease serology
- Autoimmune and immunodeficiency disorders
- Immune system principles and components
- Immunologic testing methodologies
Clinical Chemistry20-25%- Endocrinology and therapeutic drug monitoring
- Electrolytes, acid-base balance, and blood gases
- Carbohydrates, lipids, proteins, and enzymes
- Toxicology and liver/kidney function tests
Hematology20-25%- Red blood cell physiology and disorders
- Hemostasis and coagulation testing
- White blood cell morphology and diseases
- Routine and automated hematology procedures
Laboratory Operations5-10%- Pre-analytical, analytical, and post-analytical processes
- Laboratory safety and regulatory compliance
- Quality control and quality assurance
- Laboratory mathematics and instrumentation
Blood Banking (Immunohematology)15-20%- Transfusion reactions and adverse events
- Compatibility testing
- Blood components and storage
- Blood group systems and antigens
- Antibody detection and identification
Urinalysis and Other Body Fluids5-10%- Semen and amniotic fluid testing
- Physical, chemical, and microscopic analysis
- Urine formation and composition
- Cerebrospinal, serous, and synovial fluids

ASCP MEDICAL LABORATORY TECHNICIAN - MLT(ASCP) Sample Questions:

1. Which specimen should be collected last?
Question options:

A) Clotted specimen
B) Specimen requiring special handling
C) STAT specimen
D) Fasting specimen


2. Elevation in conjugated bilirubin is most likely to be found in which of the following conditions:

A) Erythroblastosis fetalis
B) Transfusion reactions
C) Biliary obstruction
D) Cirrhosis of the liver


3. Hemoglobin electrophoresis uses an electric field to separate hemoglobin molecules based on differences in net electrical charge. The rate of electrophoretic migration is also dependent on the ionic radius of the molecule, the viscosity of the solution through which it is migrating, the electrical field strength, temperature, and the type of supporting medium used.
Electrophoretic separation of hemoglobin fundamentally relies on:

A) Shape variations of molecules
B) Size differences of molecules
C) Concentration differences of molecules
D) Net charge differences of molecules


4. Which of the following legislation was developed to protect the privacy of all patient information in the health care?

A) Health Insurance Portability and Accountability Act (HIPPA)
B) Occupational Safety and Health Administration (OSHA)
C) Clinical Laboratory Improvement Amendments (CLIA)
D) Clinical Laboratory Standards Institute (CLSI)


5. To obtain a serum sample for a stat test on a patient receiving anticoagulant therapy, the recommended tube is:
Question options:

A) yellow/gray or orange
B) red/gray or gold
C) red
D) light green


Solutions:

Question # 1
Answer: C
Question # 2
Answer: C
Question # 3
Answer: D
Question # 4
Answer: A
Question # 5
Answer: A

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